A Report on The Power and Limits of Power Essay Sample free essay sample

The Power and Limits of a Vision by Paul Roazen challenges the contentions of Erikson on three cardinal constructs: individuality crisis. life rhythm and psychosocial development- the rampart of Erik Erikson’s work and his bequest to psychological science and societal scientific disciplines. In this book. Roazen argues that similar to Freud. Erikson’s popular constructs and theories may non needfully keep true. Roazen challenged his constructs chiefly by oppugning the beginning and the methods by which these constructs were derived from which are largely from the personal experiences of Erikson. This paper reviews the book of Koazen on The Power and Limits of a Vision by Erik Erikson as a response to the demand to clear up the importance and the starkness of the theories of Erikson including the primary constructs in his plants. The positions and positions in Paul Roazen’s The Power and Limits of a Vision brings into inquiry the theory and constructs of the most influential psychosocial theoretician in the individual of Erik Erikson. We will write a custom essay sample on A Report on The Power and Limits of Power Essay Sample or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Roazen. a political scientist and depth psychology historiographer provided an extended reappraisal and re-assessment of Freud’s work bespeaking that depth psychology is non the ideal signifier of intervention. Chiefly. a political scientist. it should be noted that Roazen’s critic of Erikson stems from his rating of depth psychology. societal and political idea embedded in Erikson’s theories. Unlike old psychological science bookmans. Roazen started his book by following the similarities between Erikson and Freud by specifically picking up the popular constructs that are attributed to them. For Erikson. these would include the constructs of individuality crisis. life rhythm and psychosocial development- constructs that have withstood unfavorable judgments and betterments from followings and critics of Erikson. From these three constructs. The Power and Limits of a Vision centered its treatment on the geographic expedition and appraisal of Roazen on these subjects. The first major inquiry in the book was that of the credibleness of Erikson in undertaking the issue of individuality when in fact. he had disclosed his ain confusion of his ain individuality. Hence. to link and speculate and cut down human behaviour to the pursuit of happening one’s individuality harmonizing to the book would be unreal. The Power and Limits of a Vision so traces the psychosocial development in striplings which is individuality versus function diffusion in order to joint individuality crisis. Harmonizing to the theory of Erikson. adolescents’ experiences individuality crisis during this clip as a consequence of their increasing independency from their household and the increasing influence of their societal environment peculiarly their equals. The inquiry posed by Roazen roots from his critic that Erikson himself were unable to unwrap the faith of his stepfather and of his ain faith ( Judaic ) . Consequently. the book tactfully inquiries Erikson’ s inability to admit that he was an illicit kid. Roazen’s book inquiries the concealment of Erikson’s roots as a mark that he himself was unable to labor through his individuality crisis. The Power and Limits of a Vision explores this subject further and in it Roazen speaks of the denial of Erikson’s ain individuality hence. the 1 that he had may non be reliable. While the challenges that were presented by Roazen in the book points to the personal dependability of Erikson as a theoretician. the 2nd critic dwells on human behaviour and the single differences in nearing it. In the succeeding parts of the book. Roazen inquiries the fullness of Erikson’s human behaviour constructs peculiarly his geographic expeditions of experiential crisis. Harmonizing to Roazen ( 1977 ) . persons vary in their geographic expeditions of their ain being and few would contemplate on their experiential crisis. The book argues that while people are pre-occupied with about the same concerns such as consciousness. gender. etc. . the fluctuation would impart less credibleness of Erikson’s claims. However. Roazen objectively notes that Erikson did non claim the authoritativeness of his theories on personality. But instead. the restrictions of his theories lies largely in his inability to analyze several topics merely like most psychological science bookman s do now. His ain scrutiny of his experiences therefore. lends the restrictions of his ain research. The 2nd issue in The Power and Limits of a Vision has been that of Erikson’s life rhythm theory in which Roazen argued that Erikson by explaining on his theory of psychosocial development’s eight constituents. Erikson differentiated between the positive and the negative side of development. In the procedure of making so. he is recommending uniformity to the positive side of development hence. making a position quo of uniformity in psychological science. In Roazen’s term this is similar to the indorsement of political conservativism. The rigidness of this division is implied in the sameness in the experiences of persons throughout the life cycle- something that would do the single ego ambigious in order to give manner to the societal ego. In exemplifying the life rhythm theory. Erikson harmonizing to Roazen used Marthin Luther as his focal point of discussion- from his displacement in personality and in his philosophies which is manifested in the alterations on hi s phase of development. For case. the last phase of Erikson which is Generativity V Stagnation has been achieved by King which allowed him to set humanity and God every bit good as his restrictions in his philosophies. However. despite the unfavorable judgments of Roazen. the writer acknowledges the power of Erikson’s theory in finding the positive side of human behaviour. The positive geographic expedition of Erikson which is a going from Freud’s negative geographic expedition of human behaviour showed that persons can set to their societal and personal environment in order to make an equilibrium necessary to equilibrate their personalities from that of society’s norms and traditions. Consequently. Roazen responds to the averment of Erikson that society in general had given manner to the alterations in people’s psychosocial development by bespeaking that while society had given people this chance. societies including the United States has besides deprived people from showing their ain individualism and civilization through repression. As a whole Rozen provided a balanced presentation of Erikson’s theory and concepts- while admiting that Erikson’s prognostic power in human behaviour still stands today. there are some bounds to this power. In The Power and Limits of a Vision. Roazen’s primary concern was of the methods by which Erikson had devised his theories on and on its impact on the society as a whole. In making so. it is evident in The Power and Limits of a Vision was more concerned with the social effects of Erikson’s doctrines alternatively of the single geographic expedition of the mind and personality. Roazen in his decision virtues Erikson’s ability to foretell every bit good as maintain optimism of human behaviour despite the negativisim that is evident in the plants of Freud- a major influence in Erikson’s work. Contemplation and Analysis At the start of the book. one would hold the construct that Roazen is simply knocking Erikson. However. this is non the instance as Roazen provided a balanced and well-articulated inquiries on the cogency and the dependability of Erikson’s theories. For case. from the rubric entirely. The Power and Limits of a Vision presents an recognition and congratulations of the ability of Erikson’s theories to defy unfavorable judgments but at the same clip. it besides brings Forth the failings of Erikson’s plants. By and large. Erikson’s theory and cardinal constructs have stood over several critics. most. with the same inquiries as that of Roazen. Thus. Erikson had already answered some of Roazen’s inquiries in old plants. The primary concern that is new to Roazen’s The Power and Limits of a Vision is that of the concern for the society as a consequence of the predictability in human behaviour. Roazen’s book hence. provided necessary inquiries as to the fullness of the constructs of individuality and the impact of personality and human behaviour to society as explained in the psychosocial development of Erikson. However. while The Power and the Limits of a Vision credibly expressed some failings in Erikson’s theory. it failed to sufficiently weaken the claims of Erikson. First. the inability of Roazen to supply a believable critic is his absence of an alternate theory or doctrine that would be a replacement or alternate to Erikson’s theories on individuality. life rhythm and psychosocial development. For case. he foremost rejected the theories of Freud as being excessively negative and considered Erikson as excessively positive and deterministic in qualifying the development of persons. Consequently. the deficiency of supplying a concrete psycho-social paradigm has been because of the focal point of Roazen on the society. While Erikson considers the society as an of import contextual background and force of his plants. his primary focal point is that of the person and his/her behaviour. Hence. Erikson focused on an internal analysis of the individual taking into consideration the external environment and the society as of import determiners of one’s behaviour and development. However. Roazen on the other manus. as a political scientist is more concerned with the cogency and impact of these claims on the social degree. Hence. while on one point Roazen is right. its unfavorable judgments are non plenty to weaken the claims of Erikson. Second. the critics of Roazen besides relied on the dependability of the research method employed by Erikson. For case. Roazen in the first parts of the book questioned the personal life of Erikson bespeaking that Erikson may non hold accurately described the phase of striplings and in the formation of individuality because he himself was undergoing an individuality crisis due to his inability to admit his paternal roots and his faith. By oppugning the dependability of Erikson as a beginning of informations. The Power and Limits of a Vision besides puts into inquiry the authority of qualitative method as a scientific discipline. This unfavorable judgment has both its strengths and failings: on one manus. qualitative method as used by Erikson lacks the power of generalisation or fullness peculiarly because he based it on his ain experiences ; on the other manus. this method of enquiry provides the necessary deepness of analysis that is needed in order to accurately picture the persona lity and mind of an person. Mention Roazen. P. ( 2002 ) Erik H. Erikson: The Power and Limits of a Vision by Paul Roazen Free Press. 254 pages.

The Nitty-Gritty About Reduplication

The Nitty-Gritty About Reduplication The Nitty-Gritty About Reduplication The Nitty-Gritty About Reduplication By Mark Nichol Reduplication, a type of vocabulary variation that allows writers and speakers of English to indulge in the rich potential for wordplay the language so often provides, refers to any of three types of repetitive extension of sounds. (Many other languages also feature reduplication, but this post focuses exclusively on the English tongue.) Rhyming Reduplication When we talk about doing the hokey-pokey, or refer to a razzle-dazzle spectacle or a namby-pamby attitude, we’re employing rhyming reduplication, which usually serves to emphasize with a playful near duplication of a meaningful word (fuzzy-wuzzy, itsy-bitsy), though sometimes both words have meaning and the rhyming is a fringe benefit that makes the term catchier (â€Å"chick flick†). Many reduplicatives are pairings of nonsense words (fuddy-duddy, hanky-panky), and their origin is obscure, but others with seemingly meaningless elements have at least a likely etymology: Hurly-burly may stem from the kinetic term hurl, and willy-nilly goes back several centuries to any one of several possible expressions such as â€Å"Will he, nill he† (â€Å"Whether he will or he won’t†). Exact Reduplication A similar type of construction stems from efforts by adults to help children learn by repetition hence baby talk like bye-bye and choo-choo. But some exact reduplication is used disparagingly in grown-up contexts: Two popular exact reduplicatives, blah-blah and â€Å"yada yada† (spelled and repeated variously and perhaps stemming from the earlier British English slang term yatter-yatter) mock dull or meaningless speech. Ablaut Reduplication A third form, named for the word for change of vowel sounds, is exemplified by the terms chitchat and dillydally, each of which has, unlike most rhyming reduplicatives, the substantial word in the second position rather than the first. Others, like crisscross and zigzag, more transparently demonstrate that most ablaut reduplications refer to action, especially reciprocated movements or behaviors. Note that in these examples, and in most other ablaut reduplicatives, the first vowel is always an i, produced by making a close, or high sound (meaning it is achieved by high placement of the tongue) and that the second vowel is always low. Other Reduplicative Forms New reduplicative vocabulary is slow to emerge. Exceptions include occasional slang terms (â€Å"boob tube,† hip-hop) or ad hoc coinages constructed like fancy-schmancy. (The latter type actually has a name: shm-reduplication.) Speaking of fancy-schmancy, there’s also a formal name (contrastive focus reduplication) for exact reduplication employed to clarify the relationship between a variation of an archetypal meaning and the archetypal usage itself, as in â€Å"When you say ‘Dude, that’s bad,’ do you mean good-bad, or bad-bad?† Uses of Reduplication Reduplicatives are handy little items for injecting a note of whimsy or a sharp edge into fiction or nonfiction alike. They can convey humorous or sarcastic understatement (â€Å"It’s just another case of high-finance hocus-pocus,† â€Å"The wish-washy White House flip-flops again†) or serve to mock or belittle a target (â€Å"The socialite’s hoity-toity hubris just as she tripped was literally pride coming before a fall,† â€Å"Her eency-weency voice showcased her itsy-bitsy talent†). They’re also useful, however, for positive or neutral language (â€Å"The pitter-patter of little feet on the hardwood floor presaged the appearance of my preschooler,† â€Å"My explosive sneeze caused the birds to erupt from the bushes and flee helter-skelter†). But don’t use a hodgepodge or a mishmash of reduplicatives pell-mell: Make sure you know their senses and connotations, and verify whether they’re open, hyphenated, or closed compounds. Want to improve your English in five minutes a day? Get a subscription and start receiving our writing tips and exercises daily! Keep learning! Browse the Fiction Writing category, check our popular posts, or choose a related post below:Passed vs PastWhat's a Male Mistress?50 Musical Terms Used in Nonmusical Senses

Marketing planning Essay Example | Topics and Well Written Essays - 1000 words

Marketing planning - Essay Example The time when company started its business, only 20 smoothies were sold and this figure has increased up to 420,479 smoothies’ everyday which is a considerable achievement. Increasing sales, revenues and consumption of smoothies indicate that the quality of products offered by the company has been remarkable because of which Innocent Drinks is able to achieve a lot. Products offered by the company are made up of natural fruits and the quality management team makes sure that the fruits are fresh so that the quality of the products is maintained. Product life cycle is defined as the stage of the product as the life of the product increases, it moves from growth stage to maturity stage and then it starts declining (Kotler, 2009). Smoothies are at present during the growth stage of product lifecycle (Innocent). Products offered by Innocent Drinks are also at present in the growth stage and the increasing demand of smoothies represents further growth of the product. As the products offered by the company are still growing and generating substantial amount of revenues for Innocent Drinks. The market as well as the company is growing at the moment however major portion of the industry has been captured by Innocent Drinks with approximately 71% of the total market share. At a time when the market growth rate would decline, then Innocent Drinks can be considered as cash cow. Perceptual mapping shows that the products offered by Innocent Drinks are considered as of high quality and at the same time the prices of these products are believed to be relatively higher than the prices of competitors. Although some competitors are offering it at a lower price, but then the quality of products offered by these competitors is a question mark thus Innocent Drink is able to provide quality products at a relatively higher price. According to Ansoff Matrix the

Characteristics of imprisonment in the nineteenth century Essay

Characteristics of imprisonment in the nineteenth century - Essay Example Characteristics of imprisonment in the nineteenth century During the 17th century, prison was any place that the offenders were held while awaiting the punishment. During the 17th century, London Bridewell house was constructed as a prototype prison that could hold the disorderly poor in order to instill the habits of work through prison labour. The 19th century witnessed the implementation of the first state prison that was the national penitentiary at Millbank in London. The prisoners were kept in separate cells, but were allowed to associate during the day. Pentoville prions was constructed in 1842 with cells measuring 13 feet long, 9 feet high and 7 feet in width and operated a separate system that entailed solitary confinement of the prisoners. Peel’s Gaol Act of 1823 had separated the prisons depending on the status of the offender. The prisoners were confined in a separate cell and were tied with rope and communication to other prisoners was impossible. Select Committee of the House of Lords recommended for the appointment of Prison Inspectorate and implementation of silent prison system. The first stage of silent system was solitary confinement for a period of up to nine months that was coupled with hard labour and prayers in the cells. The second penal stage was the provision of sleep and meals in a separate cell, but the prisoner would be allowed to work with the rest of prisoners. The third stage was consisted the conditional release on account of good conduct and constant police supervision. Under the silent system, food was monotonous, beds were replaced with hammocks in order to cause the offender sleepless nights and hard labour entailed Oakum picking in order to separate the fibres of an old ship’s ropes for reuse.... These systems entailed a lot of brutal punishment methods such as electric shocks, starvation and plugging of prisoners in boiling water. However, many boroughs decided to give up their prisons instead of incurring higher expenses of maintaining the prisoners (Emsley 89). In 1877, the prisons were nationalized and came under the control of Prison Commission. The Prison Commission was of the view that restructuring was essential in order to reduce the number of prisons and stop reoffending. The Prison Commission asserted its won control of prisons and standardised the regulations of all prisons thus leading to uniformity. Conclusion I agree with Sidney and Beatrice that the nineteenth century prison policy was ultimately ‘the fetish of uniformity’. After the passage of Prison Act of 1865, the Prison Commission implemented measures of standardizing services offered to prisoners including the quality of food, the number of labour hours and ensured that prisoners wore unifor ms while in the cells. In addition, the prisons employed new qualified personnel such as psychologists, caseworkers and welfare officers who were tasked with providing counseling services and providing treatment to the prisoners. By mid 1890s, the Prison Commission had successfully implemented uniformity, efficiency in administration and welfare services in prisons. The 1898 Prison Act gave the Secretary of State to make rules governing prisons and a Board of Independent Visitors for every convict prison was established (Emsley 113). Question (b). ‘When we look back, transportation captures our contemporary attention as a sensible, colourful and almost exciting way of dealing with serious offenders. But with the benefit of hindsight, we can appreciate

Anopheles Stephensi Tissue-restricted Expression

Anopheles Stephensi Tissue-restricted Expression Tissue-restricted expression and alternative splicing revealed by transcriptome profiling of Anopheles stephensi Sreelakshmi K. Sreenivasamurthy1,2, Anil Madugundu1,3, Arun Kumar Patil1,4,5, Gourav Dey1,2, Ajeet Kumar Mohanty6, Manish Kumar1,2, Krishna Patel1, Charles Wang7, Ashwani Kumar6, Akhilesh Pandey1,8,9,10,11, T. S. Keshava Prasad1,2,4,* Abstract The sequencing of Anopheles stephensi, a major malaria vector in Asia has led to increased research activity to understand the vectorial ability of this mosquito species. However, tissue-based gene expression profiles of the annotated genes remain to be understood. In this study, we summarize the transcriptomic profile of four important organs of a female imago Midgut, Malpighian tubules, Fat body and Ovary. We identified over 21,000 transcripts in total, from all the four tissues corresponding to about 12,000 gene loci. This study provides an account of the tissue-based expression profiles of majority of annotated transcripts in An. stephensi genome and alternative splicing in these tissues. Understanding of the transcript expression and gene function at the tissue level would immensely help in enhancing our knowledge of this important vector and decipher the putative role of these mosquito tissues, providing the basis of selection of candidates for future studies on vectorial abil ity. Keywords: Mosquito, RNA-seq, differential expression, lncRNAs Introduction Malaria remains as one of the most debilitating mosquito-borne diseases till date. According to WHO World Malaria Report in 2016, there were ~212 million malaria cases in the year 2015, resulting in an estimated death of about 429,000 individuals globally. Most of these cases (90%) is in the African region with about 7% incidence in South East Asia. About 50% of the Asian malaria incidence and deaths has been in India1. The number of deaths attributed to malaria in India has been reported to be higher than the WHO estimates2. However, the latest updates on the cases and deaths reported in India has been limited to the National Vector Borne Disease Control Programme (NVBDCP), according to which there has been about a million cases of malaria reported in the year 2014 [http://www.nvbdcp.gov.in/malaria3.html]. Out of the 41 different Anopheline species reported as significant vectors for transmission of human malaria, An. stephensi is an important vector in India and South Asia3,4. Bein g the major urban vector, it is second most prevalent in India. It has been reported all over the country except the north-eastern states of Sikkim, Arunachal Pradesh, Mizoram, Nagaland, Manipur and Tripura5. Sequencing of the Anopheles mosquito genomes have resulted in a spurt of activity in the study of Anopheles mosquitoes. PubMed search with the keyword Anopheles resulted in 14,576 publications, majority of which have been after the year 2000 as shown in the Figure 1A. Majority of the studies post-genome sequencing has been focussed towards understanding the role of various genes and development of numerous methods to regulate their expression.   The overall aim of the community is to embark on a feasible means to control the spread of infectious organisms either by controlling the vector/mosquito population or by curbing or reducing their vectorial ability. In this regards, numerous studies have already been performed on the recently sequenced malarial vectors6-9. However, most of the studies are focussed on previously studied molecules with very few studies focussing on new target molecules. This could probably be due to the lack of reliable data owing to incomplete genome as semblies and annotations in the identification of such targets. We have tried to bridge this gap with a huge effort of supplementing the current efforts using an integrated approach of utilizing proteomic and transcriptomic data in the genome annotation and assembly in an array of organisms through our previous studies10-12. Although transcriptomic data played a major role in refining the annotations and assembly of the genomes in the previous study, the tissue-based expression profiles were not focussed on. The tissue-based expression profiles of the identified transcripts are the focus of this study. Tissue-based expression profiling in An. stephensi has been limited to salivary glands13, ovaries14,15, testes16 and hemocytes17 with most the studies being done on whole mosquitoes14,18,19. However, even with the availability of transcriptome data from ovaries, there are several issues. The study was mainly focused on identification of transcripts expressed in developmental stages and is of low throughput14. The other tissue-based expression studies published including one cDNA-based study of the salivary glands and another being cDNA sequencing of the transcripts from hemocytes, both tissues that were not included in our study. The focus of our study is on the Midgut, ovary, Malpighian tubule and fat body of a female An. stephensi imago. These tissues, along with the salivary glands play a very important role in the blood meal digestion and thus important for the life cycle of the mosquito vector and plasmodium species. However, we restrict ourselves to understand the molecular dif ference between these mosquito tissues in the uninfected sugar-fed state of the mosquito which we believe will provide the much-needed basic understanding of the role played by these tissues. To this extent, we performed comparative and deep transcriptomic analysis of these four tissues. Materials and Methods RNA isolation and sequencing Adult female An. stephensi mosquitoes grown at the NIMR field station, Goa, were dissected to obtain midgut, Malpighian tubules, ovaries and fat body. These dissected tissues were stored in RNAlater to preserve the RNA quality till RNA extraction. The RNA isolation and sequencing was performed as described earlier10,11. Briefly, the RNA isolated using Qiagen miRNeasy kit was used for the preparation of indexed RNA-seq libraries using TruSeq RNA Sample Preparation Kit v3. The indexed and pooled libraries were sequenced on two lanes (as technical replicates) of Illumina HiScan SQ platform. Read alignment and transcript assembly The raw reads were processed for quality filtration to remove ambiguous bases present due to the sequencing errors at the 3 end of the reads. Base quality filter of >20 was considered as good. FastQC (Version 0.10.1) tool was used to determine the quality of the raw data and poor quality calls with Phred score An. stephensi genome build (ASTEI2) downloaded from VectorBase (https://www.vectorbase.org/) using HISAT (Version 2.1.0)20 aligner with the default parameters. HiSAT2 was supplied with known annotations and Gene Transfer File (GTF), AsteI2.2 from VectorBase. The alignment of reads from each lane for each tissue was carried out individually against the reference genome resulting in eight different Binary Alignment Map (BAM) files. The .bam files for each tissue were then merged to obtain merged .bam files, one for each tissue. The aligned reads were assembled against the AsteI2.2 gene annotations, as reference, using the StringTie (version 1.2.1) assembler21. Assembled transcrip ts were further quantified and annotated into known and novel categories using the gffcompare in StringTie package as described earlier22. To determine novel transcripts as a transcript GTF file and all the StringTie assemblies were merged using StringTie-merge option. Novel isoforms and intergenic transcripts were obtained by comparing the merged StringTie assemblies of all the four tissues to the annotated transcripts from VectorBase using gffcompare. Coding potential of the identified transcripts was predicted by the use of the Coding Potential Assessment Tool (CPAT)23. Transcripts which were >200 bp in length with a CPAT score threshold of Identification of differentially expressed genes across four tissues Merged GTF file from StringTie was annotated in to different classes of transcripts using gffcompare with respect to the VectorBase annotations. Expression levels of transcripts as determined by the StringTie assembler were compared across tissues. The expression information from individual lanes were used as technical replicates for each tissue. Differential expression was computed using Cuffdiff after normalizing the data across samples by calculating Fragments per Kilobase of exon per Million Fragments Mapped (FPKM)24. The R-package version 2.16.0 of cummeRbund was used for visualization, analysis of RNA-seq data and cluster generation25. An overview of the analysis pipeline is provided in Figure 1B. To identify tissue specific transcripts, we initially filtered transcripts with FPKM value à ¢Ã¢â‚¬ °Ã‚ ¥ 1.0 in at least one among the four tissue types. We then applied the right-tailed t-test to identify the transcripts which are relatively high in abundance in one tissue as again st other tissues. Results and Discussion Transcriptome sequencing of four An. stephensi tissues Midgut, Malpighian tubules, Fat body and Ovary was performed to create a tissue-based expression profile. In total, about 500 million paired-end reads of 100bp were generated from all the four tissues, with about 55 million read pairs per tissue sample from two lanes. The expression levels of transcripts between the replicates and among the tissues were comparable. Figure 2A represents the inter-tissue and intra tissue transcript expression variations in the form of a distance-based heatmap. The variations are minimal between the replicates as expected and increases between the tissues with Ovary and Malpighian tubules being the most different. By following the standard alignment and assembly pipeline using the HiSAT2 and StringTie assembler, we identified a total of about 25,000 transcripts. However, after the initial filtering for the FPKM values (à ¢Ã¢â‚¬ °Ã‚ ¥ 0.1) only 21,500 transcripts were retained. The expression of th ese transcripts was comparable across tissues with the median FPKM value ranging about 2 to 3 in all the tissues as represented by the box plot in Figure 2B. Figure 2C and 2D provides the general distribution of the length and the FPKM values of the transcript assemblies across the four tissues. About 60% of the transcript assemblies were found to have FPKM value of 1 and above, while the average length of majority of the transcripts tend to be in the range of 1000 to 3000 bp. This shows an expected trend of a reliable depth and absence of any skewing. The Transcript assemblies were classified into different classes using gffcompare. However, in order to avoid over interpretation of the data we have only focused our findings on the known =, alternate j and intergenic unknown u class of the transcript assemblies for our analysis. In our analysis, we noticed that almost equivalent number of transcript assemblies were classified under the known (=) and the alternate (j) categories. In fact, the transcript assemblies in the j category exceeded the number of known transcript assemblies. A deeper look in to this matter showed us that due to the poorly annotated gene models (which is mostly based on the prediction program) for this strain, the untranslated regions (UTRs) of the predicted transcript models in the current annotation is missed. As a result, the transcript assemblies with the extension of the exonic regions supported by the reads, probably into the UTRs were classified as alternate transcripts. We are working closely with the VectorBase to improve the annotations of these predicted gene and transcript models for the An. stephensi Indian strain. Tissue restricted transcripts Majority of the transcripts identified (about 87%) were expressed largely at similar levels in all the four tissues, the remaining 15% of the transcripts identified seemed to have more of a tissue restricted expression. Figure 3 details the distribution of the transcript expression (expressed with FPKM values à ¢Ã¢â‚¬ °Ã‚ ¥ 0.1) among the previously annotated transcripts (Figure 3A), alternative isoforms (Figure 3B) and novel previously unannotated intergenic transcripts (Figure 3C). The majority of the transcripts in each of these groups are expressed in all the four tissues with only about 3 4% of the transcripts showing tissue restricted expression. Among the known/annotated transcripts identified, 241 were found to be exclusive to Midgut, 221 exclusive to Malpighian tubules, 479 transcripts in Ovary and 436 in Fat body. The distribution of tissue specific transcripts was similar in the alternative isoforms and novel intergenic transcripts of these four tissues with 61, 67, 146 and 77 isoforms exclusively identified in Midgut, Malpighian tubules, Ovary and Fat body. In general, there was a clear bias in the number of transcripts and transcript isoforms that were common between midgut and Malpighian tubules and similarly between fat body and ovary than amongst the others. The diversity of the transcripts identified was found to be maximal in Ovary with most the transcripts being identified in this tissue, followed by fat body. Midgut had the minimal number of transcripts identified, however, the expression levels of these transcripts, in terms of FPKM, were higher than that of other tissues. Novel splice variants and their expression Apart from the known/annotated transcripts, we identified a plethora of spliced (exon-exon) reads that were not previously annotated. Assembly of such reads along with the intra exonic reads led to the identification of >8500 transcripts that were spliced differently. These alternatively spliced isoforms represent the complexity of the transcript forms and their expression in the four tissues. A summary of the differential expression of these alternate isoforms is provided in Figure 3B. As in the case of annotated transcripts, most of the alternatively spliced forms were also expressed in all the four tissues. Only about 1-2% of the total alternate transcripts isoforms were found to have tissue restricted expression. Transcript isoforms were enriched maximally in Ovaries compared to any other tissue. With 146 isoforms restricted to ovaries, it showed the highest variation in the spliced forms among the four tissues although the FPKM values for these were comparatively lower than that of other tissues. Fat body had the least representation of the alternate isoforms. The splice variants identified included examples of intron retention, alternative 3 or 5 donor and acceptor sites, exon skipping and others. Different spliced forms were expressed in different tissues. An example of transcript expressed in different tissues is provided in Figure 4. The annotated gene ASTEI04270 belongs to the Gelsolin/Vilin/fragmin superfamily, coding for a single transcript isoform according to the VectorBase annotation. However, we identified six different isoforms for the gene. The original protein coded by the annotated transcript with a signal peptide and nine gelsolin-like domains that was highly expressed in Fat body followed by Malpighian tubules. The alternative isoforms included a shorter transcript encoded by the first 3 exons (ANSTF.3986.4), which retained only three of the nine gelsolin-like domains along with the signal peptide sequence that was highly expressed in fat body and least expression in ovaries. The other 4 isoforms encoding the exons from fo urth exon consists of 4 gelsolin-like domains. Isoforms ANSTF.3986.1 and ANSTF.3986.2 were highly expressed in midgut followed by Malpighian tubules but not identified in fat body and ovaries. Whereas, isoforms ANSTF.3986.5 and ANSTF.3986.6 were significantly expressed only in midgut. Proteins encoded by this superfamily typically consists of three to six gelsolin-like domains (GEL), with each domain playing a critical role in actin filament remodeling26,27. Novel intergenic transcripts In addition to annotated and alternate spliced forms of the transcripts in the known/annotated gene loci, we found additional loci in the genome of An. stephensi Indian strain. The reads mapping to these unannotated regions were processed to assemble putative transcripts that were categorized as novel/unannotated transcripts. We identified about 2700 transcripts with FPKM values above 0.1 in the intergenic regions of the genome that were previously considered to be non-transcribed. The expression of most of these intergenic transcripts were found to be similar in all the four tissues. However, Expression-based clustering and functional correlation Since An. stephensi genome was recently sequenced and is relatively less worked upon, there is limited information on the function of these genes and transcripts. However, Gene Ontology analysis based on their translated protein and the domain structures (Interpro domains) showed that most of the differentially expressed transcripts were found to have expected domains as per the perceived function of these respective tissues. The identified transcripts were segregated into clusters based on their expression levels in the four mosquito tissues. Among the various clusters generated using the cummerbund package, few of the clusters showed clear trends of expression. One of the clusters with about 950 transcripts showed similar expression in all the four tissues. Gene level ontology mapping of these transcripts showed that majority of the transcripts possessed generic domains such as protein, nucleotide and ion binding domains, transmembrane transport, proteolysis, oxidoreductase activity and signal transduction (Figure 5A). Transcripts found to be enriched in the Midgut (170) compared to other tissues were found to have proteolytic, protein binding, hydrolase and peptidase activity. Some of the midgut enriched transcripts were found to be involved in chitin and carbohydrate metabolism (Figure 5B). Transcripts enriched in Malpighian tubules (116) were found to be associated largely with transmembrane transpor tation, oxidation-reduction process, protein and ion binding events. Few of the transcripts were associated with transferase, ligase and lyase activities among other catalytic activities (Figure 5C). Ovary enriched transcripts (241) were associated more with the protein binding, nucleic acid and ATP binding, in addition to those having signaling domains and transport domains associated with intracellular signal transduction processes such as GPCR activity, protein phosphorylation and dimerization. As expected, these transcripts seem to be involved highly in cell cycle processes including DNA replication, microtubule organization, DNA repair and growth factor activities, which are crucial mechanisms for vitellogenesis (Figure 5D). Fat body enriched transcripts (170) were consistent with the role of fat body akin to the vertebrate liver. The transcripts enriched in fat body are associated majorly with transmembrane transportation, oxidation-reduction process, chitin binding and metabo lism, heme-binding and transport, in addition to oxidoreductase activities (Figure 5E). Identification and expression of long non-coding RNAs We compared the list of transcripts identified in our study to the list of transcripts that are annotated as non-coding RNAs in VectorBase. However, we failed to identify any of the annotated non-coding RNAs in our study since the annotated ones are largely rRNAs and other small ncRNAs. Due to the ribosomal RNA depletion employed in our study, we expected no rRNAs to be identified. However, in order to investigate the presence and expression of the long non-coding RNAs in An. stephensi, we assessed the coding potential of all the identified transcripts using the CPAT tool. From this, we identified 4,071 transcripts that satisfied the criteria for the long non-coding RNAs (lncRNAs) (Supplementary Table 2). That is, they were longer than 200 bases in length and were predicted to have a coding potential of Tissues considered in this study play an important role in the life cycle of the female mosquito. They are critical in blood meal digestion, metabolism, vitellogenesis, excretion, immunogenesis, Plasmodium sporogony and reproduction, which are associated with vector physiology, progression and malaria transmission. Mosquito midgut is involved in the initial storage and digestion of the ingested blood. The gut epithelium also provides site for development of oocysts and sporozoites (Sporogony). Blood meal induces pathways such as TOR, which ultimately leads to synthesis of proteins required for egg development. Fat body and ovary are known to be involved in the utilization of the nutrients from blood to enable vitellogenesis. Malpighian tubules are known to play an important role in the mosquito xenobiotics. Fat body cells (trophoblasts) and recently, Malpighian tubules have also been shown to be involved in the immune responses28-31 and is now being considered as targets for mosquito control28,31. Towards this end, we further evaluated the expression of genes previously reported to be involved in the vector-pathogen interactions32 across the four tissues (Table 2). The affordability and accessibility of sequencing-based techniques have resulted in numerous transcriptome-based studies even in An. stephensi14,15,17,19. However, due to the low depth of the other existing studies, no significant comparison could be performed between the transcript expression from our study to that of the other studies. We deciphered the genes reported to be involved in immunity14 and evaluated the expression information for the annotated transcripts and the novel alternate isoforms across the tissues (Supplementary Table 4). Although, there has been a recent study of the cDNAs from hemocytes, we could not compare the genes expressed in their study since hemocytes were not part of our study. Another reason for non-comparison was normalization issues caused by 36bp single end reads in their study, with only 49% of it mapping to the VectorBase assembly. We provide the deepest tissue-based transcriptome profiling for these four organs of An. stephensi (Indian strain), so far. Studies such as ours depicting the transcript variations amongst tissues in its physiological states provide important baseline information. In light of such information, analysis of gene expression data in the context of changes due to blood meal, infection of insecticide resistance might lead to new perspectives and insights. This, in turn, will facilitate the choice of novel targets for vector control and transmission blocking studies and other experiments as evidenced in An. gambiae33. Data Availability The RNA-sequencing data has been submitted to the Sequence Read Archive (SRA) from NCBI and can be accessed using the project accession number SRP043489. Supplementary data Supplementary data are available at www.dnaresearch.oxfordjournal.org. Funding This paper is funded by the joint research project to NIMR and IOB entitled Characterization of Malaria Vector Anopheles stephensi Proteome and Transcriptome (EMR/2014/000444) from the Science and Engineering Research Board (SERB), Government of India. SKS and GD has been supported by the Senior Research Fellowship by University Grants Commission (UGC) and MK was supported by the Council of Scientific and Industrial Research, Government of India during the study. Table 1. Transcript distribution number of transcripts in total, class code-based classification of transcripts in all four tissues and in individual tissues      Ã‚   All 4 tissues Midgut Malpighian tubule Ovary Fat body Total number of transcripts identified 21,500 17,461 18,812 18,616 18,685 Corresponding gene location identified 12,256 10,357 11,107 10,973 11,371 Total number of known/annotated transcripts = 9,722 7,508 7,883 8,001 8,015 Number of alternate isoforms/transcripts j 8,820 7,603 8,232 7,992 8,037 Number of novel transcripts (intergenic) u 2,694 2,136 2,458 2,396 2,398 Figure Legends: Figure 1. A. Graphical representation of the remarkable increase in the number of studies on Anopheles mosquitoes post genomic era. B. Workflow representation of the study pipeline followed. Figure 2. Overall representation of transcript expression. A. HeatMap representation of the Jensen-Shannon (JS) divergence between the different tissues and their technical replicates. B. Bar-chart representation of the tissue-based transcripts and their median expression in the log10(FPKM), showing normalized distribution. C. FPKM distribution curve of the transcripts identified in the four tissues. D. Distribution of transcript length across the four tissues. Figure 3. Venn diagram representation depicting the overlap and the tissue specific expression of the transcripts across the four tissues A. For VectorBase annotated transcripts. B. Distribution of alternate isoforms of transcripts. C. Distribution of novel intergenic transcripts. Figure 4. An example representing the novel spliced forms of the VectorBase annotated gene ASTEI04270. Isoforms identified due various splicing events and their expression across the four tissues. Figure 5. Expression-based transcript clusters and the functional enrichment of the classes of transcripts based on domain and Gene Ontology-based functional annotation. A. Transcripts having similar expression in all four tissues B. Midgut-enriched transcripts C. Transcripts overexpressed in Malpighian tubules D. Transcripts highly expressed in Ovary E. Fat body-enriched transcripts.

Qantas Cas Notes Essay

Analyse and interpret data Maintain Qantas/Jetstar’s combined domestic market share of 65% Match capacity with demand sustaining loads around 80% Grow Jetstar in Asia Increase internet sales Increase customer service standards Enhance complementary portfolio businesses like Freight Reduce losses of Qantas international Transform Qantas International for focusing on right aircraft, right route, network optimisation and margin improvement Grow their frequent flyer program members and partners Market Segmentation and Selection of the Target Market Qantas’ market segmentation is complex because each segment has distinctive and different needs and expectations, such as the need to make stop-overs, the ability to pay fare levels and expectations in terms of in-flight service and comfort. Qantas mainly uses behavioural segmentation to select its target markets. Buyers are distinguished according to trip purpose e. . business and leisure/non-business travellers. Marketing Strategies Positioning Formulating the Marketing Mix Product Strategies Scheduling features Comfort-based features I ne Qantas Frequent Hyer scneme (H- Intangible benefits Brand name Price Cost plus margin: Qantas determines the cost of production and then adds a margin for profit Market: most fares at Qantas are determined by the market, where demand is matched with supply Competition based: monitoring what other airlines such as Virgin Blue are charging Pricing Strategies Price penetration Full Fares Promotional Fares Loss Leading Promotiono Advertising Sales promotions particularly in periods of subdued demand. When Qantas first launched Jetstar it released 100,000 tickets at $49. Qantas also launches a two-for- one ticket sale, which allowed a second passenger to fly for the cost of taxes and charges. Personal selling Publicity Place/Distribution Distribution to end customers is achieved by Qantas in two ways, direct and indirect. DIRECT – via its own retail outlets INDIRECT – via sales agents People Because most customers have direct contact with Qantas employees, especially on he ground, the impression they give has a big impact on how Qantas is perceived. Qantas staff must have appropriate personal attributes and training for their Jobs. Qantas spends more that $275 million a year on staff training to ensure a very positive interaction between its customers. Processes Booking flights online Online check in Mobile check in cnecK In KIOSK Physical Evidence Qantas’ customers are influences by Qantas’ signage, its website, its terminals, its lounges, etc. E-Marketing uses an electronic medium to perform marketing activities, e. g. website, email. Global Marketing is the use of marketing activities across national boundaries. Qantas uses: Global Branding Standardisation Customisation Implementing, Monitoring and Controlling the Marketing Plan Qantas has a systematic base for continually monitoring, controlling and adjusting its marketing activities using the following tools: Developing a financial forecast of revenue using statistical models, past sales data, executive Judgement and surveys of customer buying intentions. It then estimates costs such as market research costs, promotion costs, product development costs and distribution costs. Comparing actual and planned results using a number of performance criteria.

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